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Stem primers' effect on amplification reaction sensitivity has not been .. as with both loop primers, despite the drop in reaction speed (Figure ​(Figure4B4B). . these LOD values with statistical significance we had to test 99 PCR wells for .. Anal. Chem. 1980;52:2242–2249. [Google Scholar]. 37. Doyle T.

Along conventional dual labelled probes (DLP), alternative primer and probe chemistries, but has been reported to be capable of higher sensitivity and precision due to the . dPCR experiments were performed using the 37K IFC Digital Arrays of the . The statistical analysis of the measurement data was performed by.